Characterization of the binding of Actinomyces naeslundii (ATCC 12104) and Actinomyces viscosus (ATCC 19246) to glycosphingolipids, using a solid-phase overlay approach.
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Characterization of the binding of Actinomyces naeslundii (ATCC 12104) and Actinomyces viscosus (ATCC 19246) to glycosphingolipids, using a solid-phase overlay approach.
Actinomyces naeslundii (ATCC 12104) and Actinomyces viscosus (ATCC 19246) were radiolabeled externally (125I) or metabolically (35S) and analyzed for their ability to bind glycosphingolipids separated on thin layer chromatograms or coated in microtiter wells. Two binding properties were found and characterized in detail. (i) Both bacteria showed binding to lactosylceramide (LacCer) in a fashion...
متن کاملBinding of Actinomyces naeslundii to glycosphingolipids.
The type 2 fimbrial lectin of Actinomyces naeslundii WVU45 mediated the binding of this bacterium to glycosphingolipids chromatographed on thin-layer silica gel plates. Radioiodinated bacteria attached to GM1, GD1b, and globoside. After chromatograms were treated with sialidase, the bacteria also bound to GD1a and GT1b. The actinomyces lectin apparently recognized the Gal beta 3GalNAc termini o...
متن کاملMannose-contaminating agglutinin for Actinomyces viscosus and Actinomyces naeslundii.
Rapid agglutination of Actinomyces viscosus and Actinomyces naeslundii cells by D-mannose solutions was observed during studies of their attachment to mammalian cells in vitro. The specificity of the agglutination reaction was studied by slide agglutination tests and by measuring the rate of decrease in optical density of bacterial phosphate buffer suspensions caused by the setting of bacterial...
متن کاملNovel antigens of oral Actinomyces species prepared from a cell wall enzyme lysate.
Purified cell walls of Actinomyces viscosus and Actinomyces naeslundii were solubilized by the enzymatic action of Ml N-acetylmuramidase. Soluble cell wall carbohydrate antigens were purified by column chromatography. A purified antigen preparation (fraction C) from A. viscosus ATCC 19246 was found to contain deoxyhexoses (rhamnose and 6-deoxytalose), hexosse (galactose, glucose and mannose), a...
متن کاملEstablishment and distribution of Actinomyces viscosus and Actinomyces naeslundii in the human oral cavity.
The intraoral establishment and proportional distribution of suspected periodontal pathogens Actinomyces viscosus and Actinomyces naeslundii were studied using a recently developed differential plating medium, CNAC-20. Saliva and dental plaque samples were collected from 108 subjects ranging in age from infants to young adults; tongue and buccal mucosa samples were collected from only the adult...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1990
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)38584-9